Nikolaos Rousis ~ Post Doctoral Researcher

Rousis Nikolaos
Post-doctoral Researcher (Project coordinator, European Commission)
based at National and Kapodistrian University of Athens, Greece
and University of Queensland, Australia
Phone: +302107274902
E-mail: nrousis@chem.uoa.gr

Download CV in pdf

Education

B.Sc. in Chemistry (09/2005-07/2010), National and Kapodistrian University of Athens, Greece, Grade: 7.09/10
M.Sc. in Chemical Analysis & Quality Control (10/2010-09/2012), National and Kapodistrian University of Athens, Greece, Grade 9.07/10
Ph.D. in Environmental Chemistry (10/2013-07/2017), IRCCS Istituto di Ricerche Farmacologiche “Mario Negri”, Milan, Italy
Post-Doctoral research in Analytical / Environmental Chemistry (2020-today), University of Queensland, Australia and National and Kapodistrian University of Athens, Greece

Research topics

Undergraduate Thesis: “Evaluation of different modifiers for the determination of arsenic in leachate samples from sanitary landfills by electrothermal atomic absorption spectrometry”, Laboratory of Analytical Chemistry, Chemistry department, National and Kapodistrian University of Athens, Greece (2010).
Master Thesis: “Study of interferences in atomic mass spectrometry – Application to the determination of metalloids, transition and rare earth metals in Chios mastic gum”, Laboratory of Analytical Chemistry, Chemistry department, University of Athens, Greece (2012).
Ph.D. Thesis: “Identification and screening of biomarkers of human exposure to environmental and food toxicants in sewage”, Faculty Environmental Chemistry, IRCCS – Mario Negri Institute for Pharmacological Research, Milan, Italy (2016).
Post-doctoral Research: The innovative wastewater-based epidemiology approach with the advances of high resolution mass spectrometry as a complementary biomonitoring tool for assessing the health status of a population.

Workshops

  • “Method Validation and Quality Control” Research Institute for Pesticides and Water, University Jaume I, Castelló de la Plana, Spain (29-30/04/2015).
  •  “Assessing Human Health and Lifestyle by Sewage Epidemiology” IRCCS – Mario Negri Institute for Pharmacological Research, Milan, Italy (16-18/09/2014).   “Analytical Techniques for Biomarkers Analysis” KWR Water cycle Research Institute, Utrecht, the Netherlands (10-11/04/2014).
  •  “Sampling, Sample Handling Storage and Sample Preparation” Norwegian Institute for Water Research, NIVA, Oslo, Norway (09-10/09/2013).
  •  “Human Health and the Environment” University of Bath, Bath, England (22-24/04/2013).

Research Interests

General research interests:

  • Wastewater-based epidemiology
  • Development and validation of analytical methods
  •  Chemometrics and statistical analysis
  •  Human and environmental risk assessment
  •  Uncertainty measurements
  •  Interlaboratory studies: Quality assurance/Quality control (QA/QC)
  •  Participation in European projects: management, dissemination, experimental part
  •  Extractable and leachable studies (pharmaceuticals and medical devices)
  •  Separation techniques: LC-ESI-MS/MS (QqQ), UHPLC-QToF-MS, LC-LTQ-Orbitrap-MS, GC-MS,Chiral analysis
  • High Resolution Mass Spectrometry (HRMS)
  •  Atomic (mass) spectrometric techniques: ICP-MS, ETAAS, ICP-OES
  •  Contaminants: pharmaceuticals, emerging pollutants, pesticides, illicit drugs, new psychoactive  substances, metals

Projects:

  • 2020-Present: The innovative wastewater-based epidemiology approach with the advances of
    high resolution mass spectrometry as a complementary biomonitoring tool for assessing the health
    status of a population, NTS-EXPOSURE [grant agreement 896141, MSCA-IF-GF project] (European Commission)
  • 2013-2016: A new paradigm in drug use and human health risk assessment: Sewage profiling at
    the community level, SEWPROF [grant agreement 317205, MC ITN project] (European
    Commission)

Publications in journals and books

  1. N.I. Rousis, E. Gracia-Lor, M.J. Reid, J.A. Baz-Lomba, Y. Ryu, E. Zuccato, K.V. Thomas and S. Castiglioni. “Assessment of human exposure to selected pesticides in Norway by wastewater analysis” Science of the Total Environment, 2020, 723, 138132.
  2.  E. Castrignanò, Z. Yang, E.J. Feil, R. Bade, S. Castiglioni, A. Causanilles, E. Gracia-Lor, F. Hernàndez, B.G. Plósz, P. Ramin, N.I. Rousis, Y.Ryu, K.V. Thomas, P. de Voogt, E. Zuccato and B. Kasprzyk-Hordern. “Enantiomeric profiling of quinolones and quinolones resistance gene qnrS in European wastewaters” Water Research, 2020, 175, 115653.
  3.  L. Fallati, S. Castiglioni, P. Galli, F. Riva, E. Gracia-Lor, I. González- Mariño, N.I. Rousis, M. Shifah, M.C. Messa, M.G. Strepparava, M. Vai and E. Zuccato. “Use of legal and illegal substances in Malé (Republic of Maldives) assessed by wastewater analysis” Science of the Total Environment, 2020, 698, 134207.
  4.  E. Gracia-Lor, N.I. Rousis (co-first author), F. Hernández, E. Zuccato and S. Castiglioni. “Wastewater-based epidemiology as a novel biomonitoring tool to evaluate human exposure to pollutants” Environmental Science and Technology, 2018, 52, 10224-10226.
  5.  D.A. Devault, S. Karolak, Y. Lévi, N.I. Rousis, E. Zuccato and S. Castiglioni. “Exposure of urban population to pesticides assessed by wastewater-based epidemiology in a Caribbean island” Science of the Total Environment, 2018, 644, 129-136.
  6.  A. Causanilles, D.R. Cantillano, E. Emke, R. Bade, J.A. Baz-Lomba, S. Castiglioni, E. Castrignanò, E. Gracia-Lor, F. Hernández, B. Kasprzyk-Hordern, J. Kinyua, A-K. McCall, A. van Nuijs, C. Ort, B.G. Plósz, P. Ramin, N.I. Rousis, Y. Ryu, K.V. Thomas and P. de Voogt. “Comparison of phosphodiesterase type V inhibitors use in eight European cities through analysis of urban wastewater” Environment International, 2018, 115, 279-284.
  7.  E. Castrignanò, Z. Yang, R. Bade, J.A. Baz-Lomba, S. Castiglioni, A. Causanilles, A. Covaci, E. Gracia-Lor, F. Hernàndez, J. Kinyua, A-K. McCall, A. van Nuijs, C. Ort, B.G Plósz, P. Ramin, N.I. Rousis, .Y Ryu, K.V. Thomas, P. de Voogt, E. Zuccato and B. Kasprzyk-Hordern. “Enantiomeric profiling of chiral illicit drugs in a pan-European study” Water Research, 2018, 130, 151-160.
  8.  E. Gracia-Lor, N.I. Rousis, E. Zuccato, R. Bade, J.A. Baz-Lomba, E. Castrignanò, A. Causanilles, F. Hernàndez, B. Kasprzyk-Hordern, J. Kinyua, A-K. McCall, A. van Nuijs, B.G. Plósz, P. Ramin, Y. Ryu, M.M. Santos, K.V. Thomas, P. de Voogt, Z. Yang and S. Castiglioni. “Estimation of caffeine intake from analysis of caffeine metabolites in wastewater” Science of the Total Environment,
    2017, 609, 1582-1588.
  9.  N.I. Rousis and N.S. Thomaidis. “Reduction of interferences in the determination of lanthanides, actinides and transition metals by an octopole collision/reaction cell inductively coupled plasma mass spectrometer – Application to the analysis of Chios mastic” Talanta, 2017, 175, 69-76.
  10.  E. Zuccato, E. Gracia-Lor, N.I. Rousis, A. Parabiaghi, I. Senta, F. Riva and S. Castiglioni. “Illicit drug consumption in school populations measured by wastewater analysis” Drug and Alcohol Dependence, 2017, 178, 285-290.
  11.  N.I. Rousis, E. Gracia-Lor, E. Zuccato, R. Bade, J.A. Baz-Lomba, E. Castrignanò, A. Causanilles, A. Covaci, P. de Voogt, F. Hernàndez, B. Kasprzyk-Hordern, J. Kinyua, A.K. McCall, B.G. Plósz, P. Ramin, Y. Ryu, K.V. Thomas, A. van Nuijs, Z. Yang and S. Castiglioni. “Wastewater-based epidemiology to assess pan-European pesticides exposure” Water Research, 2017, 121, 270-279.
  12.  N.I. Rousis, R. Bade, L. Bijlsma, E. Zuccato, J.V. Sancho, F. Hernandez and S. Castiglioni. “Monitoring a large number of pesticides and transformation products in water samples from Spain and Italy” Environmental Research, 2017, 156, 31-38.
  13.  E. Gracia-Lor, S. Castiglioni, R. Bade, F. Been, E. Castrignanò, A. Covaci, I. González-Mariño, E. Hapeshi, B. Kasprzyk-Hordernd, J. Kinyua, F.Y. Lai, T. Letzel, L. Lopardo, M.R. Meyer, J. O’Brien, P. Ramin, N.I. Rousis, A. Rydevik, Y. Ryu, M.M. Santos, I. Senta, N.S. Thomaidis, S. Veloutsou, Z. Yang, E. Zuccato and L. Bijlsma. “Review; Measuring biomarkers in wastewater-based epidemiology as a new source of epidemiological information: current state and future perspectives” Environment International, 2017, 99, 131-150.
  14.  N.I. Rousis, E. Zuccato and S. Castiglioni. “Wastewater-based epidemiology to assess human exposure to pyrethroid pesticides” Environment International, 2017, 99, 213-220.
  15.  R. Bade, L. Bijlsma, J.V. Sancho, J.A. Baz-Lomba, S. Castiglioni, E. Castrignanò, A. Causanilles, E. Gracia-Lor, B. Kasprzyk-Hordern, J. Kinyua, A-K. McCall, A. van Nuijs, C. Ort, B.G. Plósz, P. Ramin, N.I. Rousis, Y. Ryu, K. Thomas, P. de Voogt, E. Zuccato and F. Hernandez. “Liquid chromatography-tandem mass spectrometry determination of synthetic cathinones and phenethylamines in influent wastewater of eight European cities” Chemosphere, 2017, 168, 1032- 1041.
  16.  Y. Ryu, E. Gracia-Lor, R. Bade, J.A. Baz-Lomba, J.G. Bramness, S. Castiglioni, E. Castrignanò, A. Causanilles, A. Covaci, P. de Voogt, F. Hernandez, B. Kasprzyk-Hordern, J. Kinyua, A-K. McCall, C. Ort, B.G. Plósz, P. Ramin, N.I. Rousis, M.J. Reid and K. Thomas. “Increased levels of the oxidative stress biomarker 8-iso-prostaglandin F2α in wastewater associated with tobacco use” Scientific Reports, 2016, 6, 39055.
  17.  J.A. Baz-Lomba, S. Salvatore, E. Gracia-Lor, R. Bade, S. Castiglioni, E. Castrignanò, A. Causanilles, F. Hernandez, B. Kasprzyk-Hordern, J. Kinyua, A-K. McCall, A. van Nuijs, C. Ort, B.G. Plósz, P. Ramin, M. Reid, N.I. Rousis, Y. Ryu, P. de Voogt, J. Bramness and K. Thomas. “Comparison of pharmaceutical, illicit drug, alcohol, nicotine and caffeine levels in wastewater with sale, seizure and consumption data for 8 European cities” BMC Public Health, 2016, 16, 1035.
  18.  N.I. Rousis, E. Zuccato and S. Castiglioni. “Monitoring population exposure to pesticides based on liquid chromatography-tandem mass spectrometry measurement of their urinary metabolites in urban wastewater: A novel biomonitoring approach” Science of the Total Environment, 2016, 571, 1349-1357.
  19.  I. González-Mariño, E. Gracia-Lor, N.I. Rousis, E. Castrignanò, K.V. Thomas, J.B. Quintana, B. Kasprzyk-Hordern, E. Zuccato and S. Castiglioni. “Wastewater-Based Epidemiology to monitor synthetic cathinones use in different European countries” Environmental Science and Technology, 2016, 50, 10089-10096.
  20.  R. Bade, N.I. Rousis, L. Bijlsma, E. Gracia-Lor, S. Castiglioni, J.V. Sancho and F. Hernandez. “Screening of pharmaceuticals and illicit drugs in wastewater and surface waters of Spain and Italy by high resolution mass spectrometry using UHPLC-QTOF MS and LC-LTQ-Orbitrap MS” Analytical and Bioanalytical Chemistry, 2015, 407, 8979-8988.
  21.  N.I. Rousis, I.N. Pasias and N.S. Thomaidis. “Attenuation of interferences in collision/reaction cell inductively coupled plasma mass spectrometry, using helium and hydrogen as cell gases – Application to multi-element analysis of mastic gum” Analytical Methods, 2014, 6, 5899-5908.
  22.  I.N. Pasias, A.K. Psoma, N.I. Rousis and N.S. Thomaidis. “Inorganic Arsenic in Foodstuffs: Health Effects and Analytical Methods for Its Determination” in Marissa Jane Olson (Ed.), “Arsenic: Detection, Management Strategies and Health Effects”, Nova Science Publishers Inc., New York, 2014, pp. 67-89.
  23.  A.K. Psoma, I.N. Pasias, N.I. Rousis, K.A. Barkonikos and N.S. Thomaidis. “Development, validation and accreditation of a method for the determination of Pb, Cd, Cu and As in fish and fish feed samples” Food Chemistry, 2014, 151, 72-78.
  24.  V.P. Kalantzis, N.I. Rousis, I.N. Pasias, N.S. Thomaidis and E.A. Piperaki. “Evaluation of different modifiers for the determination of arsenic in leachate samples from sanitary landfills by electrothermal atomic absorption spectrometry” Analytical Letters, 2012, 45, 592-602.

Conferences Presentations

  1. . E. Gracia-Lor, N.I. Rousis, F. Hernández, E. Zuccato and S. Castiglioni. “Evaluating population exposure to food contaminants through wastewater-based epidemiology: pesticides and mycotoxins as pilot studies” 3rd International Conference on “Wastewater-based epidemiology: current applications and future perspectives” – Testing the Waters, Lisbon, Portugal, 26-27 October 2017
    (oral presentation).
    2. N. Salgueiro-Gonzalez, S. Castiglioni, E. Gracia-Lor, N.I. Rousis, L. Bijlsma, A. Celma, F. Hernandez and E. Zuccato. “Screening new psychoactive substances in urban wastewater from different European countries” 3rd International Conference on “Wastewater-based epidemiology: current applications and future perspectives” – Testing the Waters, Lisbon, Portugal, 26-27 October
    2017 (oral presentation).
    3. N. Salgueiro-Gonzalez, S. Castiglioni, E. Gracia-Lor, N.I. Rousis, L. Bijlsma, A. Celma, F. Hernandez and E. Zuccato. “Screening of new psychoactive substances in urine samples by high resolution mass spectrometry” XXI Reunión de la Sociedad Española de Química Aanalítica (SEQA), Valencia, Spain, 5-7 September 2017 (oral presentation).
    4. N.I. Rousis. “Evaluation of human exposure to pesticides based on measurements of their urinary metabolites in urban wastewater: a novel biomonitoring approach” 3rd PhD Students Meeting, Milan, Italy, 13-14 July 2016 (oral presentation).
    5. N.I. Rousis, S. Castiglioni and E. Zuccato. “Determination of biomarkers of exposure in raw wastewater by liquid chromatography-tandem mass spectrometry – Pesticides as the case study” 2nd International Conference on “Wastewater-based drug epidemiology” – Testing the Waters, Ascona, Switzerland, 11-15 October 2015 (oral presentation).
    6. N.I. Rousis. “SEWPROF – Pesticides: Analytical techniques, environmental fate and effects” Invited presentation at high school in Milan, Italy, 2014 (oral presentation).
    7. S. Castiglioni, A. Borsotti, E. Gracia Lor, N.I. Rousis, C. Martins and E. Zuccato. “A novel approach to study human habits through mass spectrometric analysis of urban wastewater” Society of Environmental Toxicology and Chemistry (SETAC) Europe 24th Annual Meeting, Basel, Switzerland, 11-15 May 2014 (oral presentation).
    8. N.I. Rousis, P. Nisianakis and N.S. Thomaidis. “Reduction of interferences in the determination of rare earth elements by an octopole collision/reaction cell inductively coupled plasma mass spectrometer – Application to the analysis of Chios mastic gum” Proceedings of the European Winter Conference on Plasma Spectrochemistry, Krakow, Poland 2013 (poster presentation).
    9. N.I. Rousis, P. Nisianakis and N.S. Thomaidis. “Attenuation of interferences in collision/reaction cell inductively coupled plasma mass spectrometry, using helium and hydrogen as cell gases – Application to multi-element analysis in mastic gum” Proceedings of the European Winter Conference on Plasma Spectrochemistry, Krakow, Poland 2013 (poster presentation).
    10. V. Kalantzis, N.I. Rousis, I.N. Pasias, N.S. Thomaidis and E.A. Piperaki. “Evaluation of different modifiers for the determination of arsenic in drainage samples from solid waste dump areas by electrothermal atomic absorption spectrometry” Proceedings of the 7th Aegean Analytical Chemistry Days, Mytilene, Greece 2010 (poster presentation).

Awards and Honors

Scholarship for PhD Research: The research project was funded by the European Union’s Seventh
Framework Programme for Research, Technological Development and Demonstration [grant agreement 317205, the SEWPROF MC ITN project, ‘A new paradigm in drug use and human health risk assessment: Sewage profiling at the community level’].

Professional Affiliations

  • Association of Greek Chemists, 2010 – Present
  •  SCORE COST Action ES1307, 2014 – Present

Contact information

Nikolaos Rousis
Office: 4
th floor, Wing D, Office 7
Phone: 210 7274902
Postal Address: Panepistimioupolis Zografou, Postal Code:15771, Athens, Greece
Email: nrousis@chem.uoa.gr