Nikiforos Alygizakis


Nikiforos Alygizakis
Ph.D. in Analytical Chemistry

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Date of Birth 05/09/1991
Place of Birth Chania, Crete, Greece
Workplace Address Laboratory of Analytical Chemistry,Department of Chemistry, University of Athens


2009 – 2013

B.Sc. in Chemistry (2009-2013), University of Athens, Grade: 9.18/10.00

2013 – 2015

M.Sc. in Chemical Analysis-Quality Control, University of Athens, Grade 9.65/10.00

2015 – 2019

Ph.D. in Analytical Chemistry, University of Athens

Research topic

  • Ph.D. Thesis
    Development of novel methodologies for the identification of unknown compounds in the aquatic environment employing high resolution mass spectrometry
    The objective of the thesis was to develop novel workflows employing state-of-the-art target, suspect and non-target screening tools and apply them on samples obtained from important European ecosystems such as the Danube River Basin (DRB) and the Black Sea (BS). More details are available at the following publications with DOI: 10.1016/j.trac.2019.04.008, 10.1021/acs.est.8b00365 and 10.1016/j.envint.2019.03.060.

  • Master Thesis
    Implementation of chemometric techniques for the detection and non-target identification  of analytes with distringuished time pattern in wastewater by LC-Q-TOF
    The aim of this proposal was to implement a computational methodology that would facilitate the detection of contaminants with high fluctuation in intensity among wastewater samples sampled in time-series. The output of this methodology was a compound table with top-prioritized components. The identity of the top-ranked components was revealed by established non-target identification workflows. More details are available at the publication with DOI 10.1016/j.jhazmat.2018.08.073.

  • Undergraduate Thesis
    Determination of drugs of abuse and their metabolites in wastewater by LC-MS/MS (QQQ)
    The objective of this study was to determine the concentration levels of illicit drugs and their metabolites in wastewater samples and the receiving environment. Concentration levels from wastewater samples were used to estimate the consumption of the drugs by the society (sewage epidemiology). More details are available at the publication with DOI 10.1021/acs.est.6b02417 and 10.1016/j.scitotenv.2015.09.145.

Research Interests

  • Automated data processing workflows for LC and GC HRMS
  • Advanced statistics and chemometrics techniques for prioritization of peaks in LC and GC HRMS
  • Identification of unknown compounds using not-target screening workflows
  • Digital archivation of LC and GC HRMS chromatograms
  • Wide-scope retrospective screening of emerging contaminants in digitally-archived samples
  • Wide-scope non-target screening for the identification of river basin specific pollutants
  • Application of bioassays to assess the biological responses of environmental samples
  • Determination of Antibiotics, Transformation Products and Antibiotic Resistant Genes (A&TPs&ARGs)
  • Application of prioritization decision trees to propose emerging contaminants to be regulated
  • Investigation of the fate of emerging contaminants in the environment
  • New emerging technologies to achieve the widest possible chemical coverage (e.g. Use of Hydrophilic interaction chromatography (HILIC) in monitoring)
  • Big data, construction of databases and interfaces

Professional Affiliations

  • Association of Greek Chemists (2013-present)
  • Hellenic Society of Mass Spectrometry (2015-present)
  • NORMAN network (2015-present)
  • COST Action SCORE ES1307 (2016-present)
  • COST Action NEREUS ES1403 (2016-present)
Scholarships - Awards
  • 2016-2019: Marie Skłodowska-Curie Fellowship at “ANSWER” Project (Grant agreement No 675530)
  • 2013-2015: Scholarship John S. Latsis Foundation for graduate studies
  • 2010-2013: Scholarship Antonios Papadakis, University of Athens (granted after exams)
  • 2011-2012 & 2012-2013: Award from University of Athens (Heritage of Pantia Ralli) for high performance among students of department of Chemistry, University of Athens
  • 2011-2012: Award State Scholarships Foundation «IKY» for academic performance during 5th - 6th semesters of undergraduate studies